C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2417233
A method of isolating polynucleotides encoding polypeptides affecting an organization of a subcellular organelle or structure of interest is provided. The method comprises: (a) expressing within a plurality of cells an expression library including a plurality of expression constructs each encoding a polypeptide of interest; (b) highlighting the subcellular organelle or structure of interest of the plurality of cells; and (c) isolating a cell or cells of the plurality of cells in which a cellular distribution and/or level of the subcellular organelle or structure of interest is altered to thereby isolate polypeptides capable of affecting the organization of the subcellular organelle or structure of interest.
L'invention concerne une méthode d'isolation de polynucléotides codant des polypeptides qui affectent l'organisation d'un organite ou d'une structure intracellulaire recherché. Cette méthode consiste à: (a) exprimer dans une pluralité de cellules une banque d'expression contenant une pluralité de produits recombinés d'expression codant chacun un polypeptide recherché; (b) marquer l'organite ou la structure intracellulaire recherché contenu dans la pluralité de cellules; et enfin, (c) isoler une ou plusieurs cellules parmi la pluralité de cellules dans laquelle la distribution cellulaire et/ou le niveau de l'organite ou de la structure intracellulaire recherché est modifié, de manière à isoler les polypeptides capables d'affecter l'organisation de l'organite ou de la structure intracellulaire recherché.
Ben-Ze'ev Avri
Bershadsky Alexander
Geiger Benjamin
Kam Zvi
Shtutman Michael
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Yeda Research & Development Co. Ltd.
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