Methods of making modular fusion protein expression products

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/74 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2626666

The invention relates to methods of making modular chimeric protein expression products and compositions utilized in the methods. In particular, the invention relates to sequential, directional cloning of polynucleotides encoding polypeptide modules. Each clonable element or module contains an open reading frame of interest flanked by predetermined restriction sites. The methods include using modules and vectors containing these modules as starting materials for recombinant DNA techniques. One advantage of the invention is that it allows for many variations of fusion proteins to be made quickly and easily without needing to design and evaluate each subsequent cloning step.

La présente invention concerne des procédés pour préparer des produits d'expression de protéines chimères modulaires, et des compositions employées dans le cadre des procédés. L'invention concerne en particulier des polynucléotides codant pour des modules polypeptidiques codant. Chaque module ou élément clonable contient une trame de lecture ouverte d'intérêt flanquée de sites de restriction prédéterminés. Le procédé comprend l'utilisation de modules et de vecteurs contenant ces modules, en tant que substances de départ pour des techniques de recombinaison d'ADN. L'invention présente l'avantage de permettre à de nombreuses variations des protéines de fusion d'être obtenues rapidement et simplement sans impliquer de conception et d'évaluation de chaque opération de clonage suivante.

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