Methods of manipulating and sequencing nucleic acid...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/63 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12N 15/66 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2388907

The present invention relates generally to methods, kits and compositions for use in manipulating nucleic acid molecules, particularly cloning, sequencing, amplifying and mutating such molecules. In particular, the invention relates to use of recombination sites and recombinational cloning to manipulate, select and analyze nucleic acid molecules of interest.

L'invention concerne de manière générale des procédés, des assemblages et des compositions utilisés lors de la manipulation de molécules d'acide nucléique, et en particulier le clonage, le séquençage, l'amplification et la mutation de ces molécules. Cette invention concerne en particulier l'utilisation de la technique de recombinaison de sites et le clonage recombinatoire employés afin de manipuler, sélectionner et analyser les molécules d'acide nucléique présentant un intérêt.

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