Methods of mhc class ii epitope mapping, detection of...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) A61K 39/385 (2006.01) C07K 1/00 (2006.01) C12N 15/09 (2006.01) C12P 21/06 (2006.01) G01N 33/50 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2440474

The present invention provides of using multimeric MHC class II/peptide complexes. In one aspect, methods provided for identifying MHC class II- restricted immune epitopes of a predetermined polypeptide antigen. Methods for identifying an immunostimulatory epitope for a predetermined polypeptide antigen are provided. In a related aspect, methods for screening a therapeutic polypeptide agent for an MHC class II epitope are provided. In other aspects, methods for modulating T cells and for determining or monitoring an MHC class II-restricted immune status of a patient are also provided.

L'invention concerne l'utilisation de complexes multimères peptidiques de classe II de CMH. Dans un mode de réalisation, l'invention concerne des procédés permettant d'identifier des épitopes autoimmuns limités à la classe II de CMH d'un antigène polypeptidique prédéterminé. L'invention concerne également des procédés d'identification d'un épitope immunostimulateur pour un antigène polypeptidique prédéterminé. Dans un mode de réalisation connexe, l'invention concerne des procédés de criblage d'un agent polypeptidique thérapeutique pour un épitope de classe II de CMH. Dans d'autres modes de réalisation, l'invention concerne des procédés de modulation de lymphocytes T pour déterminer ou surveiller un état immunitaire limité à la classe II de CMH d'un patient.

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