Methods of modulating cellular activity involving...

A - Human Necessities – 61 – K

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A61K 38/43 (2006.01) A61K 38/00 (2006.01) A61K 38/18 (2006.01) A61K 38/19 (2006.01) A61P 29/00 (2006.01) A61P 35/00 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2569687

The present invention relates generally to a method of modulating cellular activity and to agents for use therein. More particularly, the present invention provides a method of modulating cellular activity by modulating intracellular translocation of sphingosine kinase to the cell membrane. In a related aspect, the present invention provides a method of modulating sphingosine kinase mediated signalling via modulation of its intracellular translocation and agents for use therein. The present invention still further extends to sphingosine kinase variants and to functional derivatives, homologues and analogues thereof, exhibiting ablated or reduced capacity to undergo translocation. The method and molecules of the present invention are useful, inter alia, in the treatment and/or prophylaxis of conditions characterised by aberrant, unwanted or otherwise inappropriate cellular functional activity and/or aberrant, unwanted or otherwise inappropriate sphingosine kinase mediated signalling. The present invention is further directed to methods for identifying and/or designing agents capable of modulating sphingosine kinase intracellular translocation.

Procédé de modulation de l'activité cellulaire et agents utilisés à cet effet. Plus particulièrement, procédé de modulation de l'activité cellulaire par modulation de la translocation intracellulaire de la sphingosine kinase vers la membrane cellulaire. Selon une variante, on prévoit un procédé de modulation de la signalisation induite par la sphingosine kinase via la modulation de sa translocation intracellulaire et des agents utilisés à cet effet. Variantes de la sphingosine kinase et dérivés fonctionnels, homologues et analogues faisant preuve d'une capacité amputée ou réduite pour effectuer une translocation. Le procédé et les molécules de cette invention sont utilisés, entre autres, dans le traitement et/ou la prévention de conditions caractérisées par une activité fonctionnelle cellulaire aberrante, indésirable ou inappropriée et/ou une signalisation induite par la sphingosine kinase aberrante, indésirable ou inappropriée. Procédé d'identification et/ou de conception d'agents aptes à moduler la translocation intracellulaire de la sphingosine kinase.

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