Methods of nonspecific target capture of nucleic acids

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2658105

Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.

La présente invention concerne des procédés permettant de capturer un acide nucléique cible dans un échantillon au moyen d'une sonde de capture qui se lie de manière non spécifique à l'acide nucléique cible et se lie spécifiquement à une sonde immobilisée par l'intermédiaire d'une paire de liaison spécifique comportant un élément sur la sonde de capture et un élément sur la sonde immobilisée. L'invention concerne des compositions contenant une sonde de capture qui se lie de manière non spécifique à un acide nucléique cible et spécifiquement à une sonde immobilisée par la liaison d'éléments d'une paire de liaison spécifique dans une phase de la solution d'un mélange réactionnel.

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