Methods of nucleic acid isolation

Details Methods of nucleic acid isolation Methods of nucleic acid isolation

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2310782

The invention relates to new methods of RNA isolation that exploit the surprising discovery that RNA may be differentially precipitated from DNA. The subject methods result in the formation of an RNA-containing precipitate that has an RNA content at least two fold enriched with respect to DNA, as compared with the RNA to DNA ratio of the solution from which the RNA-containing precipitate is derived. By precipitating RNA from a solution, the RNA may be collected by simple procedures such as centrifugation or filtration, thereby avoiding the need to bind the RNA to solid phase. Preferred precipitate forming chaotropic salts are guanidine hydrochlore and guanidine thiocyanate.

L'invention concerne de nouvelles méthodes d'isolement de l'ARN, qui s'appuient sur la découverte selon laquelle l'ARN peut être soumis à une précipitation différentielle à partir de l'ADN. Les méthodes de cette invention permettent notamment de former un précipité contenant de l'ARN et présentant une teneur en ARN deux fois plus importante que l'ADN, en comparaison du rapport ARN-ADN de la solution à partir de laquelle ledit précipité contenant de l'ARN est dérivé. Grâce à cette précipitation de l'ARN à partir d'une solution, on peut recueillir l'ARN par des procédures simples, notamment par centrifugation ou filtration, ce qui permet d'éviter de lier l'ARN à une phase solide. L'hydrochlorure de guanidine et le thiocyanate de guanidine constituent des précipités préférés pour former des sels chaotropiques.

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