Methods of obtaining cell populations from adipose tissue

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01)

Patent

CA 2740578

Provided herein are methods of efficiently obtaining large numbers of viable, freshly isolated cells from small amounts of adipose tissue, as well as methods of enriching or selecting for target cell populations found therein. In certain embodiments, the method of obtaining a population of cells from adipose tissue comprises incubating the adipose tissue in a solution comprising an enzyme at a concentration which is at least 200 U/ml solution and not more than about 319 U/ml solution. In some embodiments, the method is devoid of any steps which expand the population of cells obtained. In certain aspects, the method further comprises positive or negative selection steps for obtaining an enriched population of target cells from adipose tissue. Related methods of preparing a pharmaceutical composition comprising cells for administration to a patient and methods of treating a disease or medical condition in a patient are further provided herein.

L'invention concerne des procédés permettant d'obtenir efficacement de grand nombre de cellules viables, fraîchement isolées, à partir de petites quantités de tissu adipeux, ainsi que des procédés d'enrichissement ou de sélection pour les populations cellulaires cibles trouvées dans celui-ci. Dans certains modes de réalisation, le procédé d'obtention d'une population de cellules à partir de tissu adipeux comporte une incubation du tissu adipeux dans une solution comportant une enzyme à une concentration qui est d'au moins 200 U/ml de solution et n'est pas supérieure à environ 319 U/ml de solution. Dans certains modes de réalisation, le procédé est dépourvu de toute étape permettant d'élargir la population de cellules obtenue. Sous certains aspects, le procédé comporte en outre des étapes de sélection positives ou négatives pour obtenir une population enrichie de cellules cibles à partir de tissu adipeux. Des procédés associés de préparation d'une composition pharmaceutique comportant des cellules pour l'administration à un patient et des procédés de traitement d'une maladie ou d'un état médical chez un patient sont en outre décrits.

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