C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07K 14/47 (2006.01) C12N 9/90 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2367723
The invention disclosed herein comprises a method of obtaining full-length coding cDNA. The invention method comprises using isolated full-length mRNA to synthesize first strand cDNA, attaching a non-native tag to the cDNA and using the tagged first strand to synthesize second strand cDNA. The final cDNA can additionally be amplified and inserted into an expression vector. Also disclosed are isolated full-length coding cDNAs prepared using the method of the invention, as well as nucleic acid constructs containing the full-length coding cDNAs.
L'invention porte sur un procédé d'obtention d'un ADNc codant pleine longueur. Ce procédé consiste à isoler un ARNm pleine longueur afin de synthétiser un premier brin d'ADNc, à fixer une étiquette non indigène sur l'ADNc et à utiliser le premier brin étiqueté pour synthétiser le second brin d'ADNc. L'ADNc final peut être ensuite amplifié et inséré dans un vecteur d'expression. L'invention porte également sur des ADNc codant pleine longueur préparés selon ce procédé, ainsi que sur des produits de recombinaison d'acide nucléique contenant les ADNc codant pleine longueur.
Comiskey John D.
Heyman John A.
Marcil Robert
Phelan Dorothy
Invitrogen Corporation
Mbm Intellectual Property Law Llp
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