Methods of obtaining optically active epoxides and vicinal...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/55 (2006.01) C07K 16/40 (2006.01) C12N 9/14 (2006.01) C12P 7/18 (2006.01) C12P 17/02 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2564119

The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific styrene epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active styrene vicinal diols and optically active styrene epoxides.

L'invention concerne des souches de levure, et des polypeptides codés par des gènes de ces souches de levure, qui présentent une activité d'hydrolase époxyde de styrène énantiospécifique. L'invention concerne également des molécules d'acides nucléiques codant de tels polypeptides, des vecteurs contenant ces molécules d'acides nucléiques et des cellules contenant ces vecteurs. L'invention concerne enfin des procédés permettant d'obtenir des diols vicinaux de styrène optiquement actifs ainsi que des époxydes de styrène optiquement actifs.

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