Methods of optimizing the secretion of protein in prokaryotes

Details Methods of optimizing the secretion of protein in prokaryotes Methods of optimizing the secretion of protein in prokaryotes

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12N 15/31 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12P 21/02 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2621608

Methods are provided for producing recombinant proteins by utilizing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the periplasm or extracellular medium. Expression vectors which encode a fusion protein comprising a carrier protein and the protein are also provided, as are host cells transformed with the expression vectors.

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