Methods of preparing feeder cells-free, xeno-free human...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 5/071 (2010.01) C12N 5/0735 (2010.01) C12N 5/02 (2006.01)

Patent

CA 2508880

The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

Cette invention concerne des procédés permettant de constituer et de propager des lignées de cellules souches embryonnaires humaines au moyen de systèmes de cultures exempts de cellules nourricières et de xéno-contaminants, et des cellules souches qui peuvent être maintenues dans un état non différencié, multipotent et prolifératif dans une culture exempte de xéno-contaminants et de cellules nourricières.

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