Methods of preserving prokaryotic cells and compositions...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/04 (2006.01) A61K 35/74 (2006.01) A61K 39/02 (2006.01)

Patent

CA 2272821

This invention provides methods of drying and stabilizing prokaryotic cells, and the compositions obtained thereby. The cells are first cultured or incubated under conditions sufficient to induce intracellular trehalose, suspended in a stabilizing solution and dried to form a solid glass. The resulting product is storage-stable at room temperature, showing little viability loss on storage.

La présente invention concerne des procédés de séchage et de stabilisation de cellules procaryotes, et les compositions résultantes. Les cellules sont d'abord mises en culture ou incubées dans des conditions suffisantes pour induire une tréhalose intracellulaire, suspendues dans une solution stabilisante, puis séchées pour former un verre solide. Le produit obtenu est stable au stockage à température ambiante, et présente une faible perte de viabilité au stockage.

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