Methods of producing peptides/proteins in plants and...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 19/00 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2553257

Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

L'invention concerne des méthodes permettant d'accroître le rendement au niveau de l'expression par les plantes de protéines recombinées consistant : à développer par génie génétique des sites de glycosylation dans des gènes clonés ou des ADNc de protéines à l'aide de codons qui entraînent des modifications post-translationnelles dans des plantes ; et à développer par génie génétique les gènes clonés ou les ADNc afin qu'ils contiennent une séquence signal de sécrétion de plantes qui cible les produits génétiques (protéine) à sécréter. Lesdites méthodes entraînent des rendements de protéines glycosylées recombinées accrus. L'invention concerne également des protéines produites selon lesdites méthodes.

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