Methods of profiling gene expression, protein or metabolite...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) A61K 45/00 (2006.01) A61P 43/00 (2006.01) C12N 15/09 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 37/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2443524

The present invention relates to methods of analysing gene expression, metabolite or protein levels, the use of such methods to generate distinctive profiles of a cell, and the use of such profiles in the diagnosis of disease and mode of action of novel compounds used in the treatment of a cell. These simplified methods of obtaining molecular profiles of a cell have the advantage that they allow the skilled man to produce a profile of the levels of a chosen class of molecules in a cell, which may be used to distinguish between different treatments or different cellular states, whilst being produced from fewer data than are typically used in the production of existing profiles.

La présente invention concerne des techniques d'analyse de l'expression génique, de détermination des niveau de métabolites et de protéines, l'utilisation de ces techniques pour l'établissement de profils distinctifs d'une cellule, et l'emploi de tels profils pour le diagnostic d'une maladie et le mode d'action de nouveaux composés pour le traitement d'une cellule. Ces techniques simplifiées offrent l'avantage de permettre au spécialiste d'établir un profil de niveau pour une classe donnée de molécules dans une cellule, profils qui peuvent être utilisés pour à faire une distinction entre différents traitements d'états cellulaires différents alors même que ces profils sont obtenus à partir d'un volume de données inférieur au volume généralement utilisé pour la détermination de profils existants.

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