Methods of protein production in yeast

Details Methods of protein production in yeast Methods of protein production in yeast

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2534352

Vectors, host cells, and methods are provided for the production of proteins in yeast. The vectors generally contain a selection gene, a yeast 2 micron sequence, and a polynucleotide encoding a polypeptide, where the polynucleotide is operably linked to promoter, and where the polynucleotide contains one or more yeast-preferred codons. Host cells are cultured under conditions where, after an initial batch phase, oxygen concentration is kept high and glucose feed is regulated so that the yeast cells stay in respiratory metabolism.

L'invention concerne des vecteurs, des cellules hôtes et des procédés destinés à la production de protéines dans une levure. Les vecteurs contiennent généralement un gène de sélection, une séquence de levure à 2 microns, et un polynucléotide codant un polypeptide, le polynucléotide étant lié, de façon coopérante, au promoteur, et le polynucléotide contenant un ou plusieurs codons préférés de la levure. Des cellules hôtes sont cultivées dans des conditions où, après une phase de charge initiale, la concentration en oxygène est maintenue élevée, et l'alimentation en glucose est régulée de façon que les cellules de levure soient en métabolisme respiratoire.

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