Methods of purifying anti a beta antibodies

Details Methods of purifying anti a beta antibodies Methods of purifying anti a beta antibodies

C - Chemistry, Metallurgy

07

K

C07K 16/18 (2006.01) A61K 39/395 (2006.01)

Patent

CA 2611815

The present application provides methods of purifying A.beta. binding proteins having a Fc region, for example, anti-A.beta. antibodies or antibody fusions, by adsorbing the A.beta. binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed A.beta. binding protein. The present application also features methods of eluting the purified A.beta. binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

La présente invention concerne des procédés de purification de protéines de liaison Aß possédant une région Fc, par exemple, des anticorps anti Aß ou des fusions d'anticorps, par adsorption des protéines de liaison Aß vers un agent de liaison Fc, tel que par exemple, une protéine A ou une protéine G, suivi par un lavage avec un tampon de sel cationique divalent afin de retirer des impuretés et de récupérer ensuite la protéine de liaison Aß adsorbée. Cette invention concerne aussi des procédés d'élution de la protéine de liaison Aß purifiée ainsi que l'incorporation de ces procédés dans un ensemble d'étapes de purification. Cette invention concerne aussi des kits comprenant des composants permettant de réaliser ces procédés et des instructions d'utilisation de ceux-ci.

Affiliated with

Also associated with

Rating

Methods of purifying anti a beta antibodies does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of purifying anti a beta antibodies, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of purifying anti a beta antibodies will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2050357