Methods of purifying fc region containing proteins

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 16/00 (2006.01) A61K 39/395 (2006.01) C07K 16/18 (2006.01)

Patent

CA 2611816

The present application provides methods of purifying polypeptides having a Fc region, for example, antibodies or antibody fusions, by adsorbing the polypeptides to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed polypeptides. The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

La présente invention concerne des procédés pour purifier des polypeptides possédant une région Fc, par exemple, des anticorps ou des fusions d'anticorps par l'absorption des polypeptides vers un agent liant Fc tel que la protéine A ou la protéine G, et pour effectuer un lavage avec un tampon de sel cationique divalent destiné à enlever les impuretés puis à récupérer les polypeptides adsorbés. La présente demande concerne aussi des procédés pour éluter le polypeptide purifié ainsi que l'intégration de procédés dans un cycle de purification. L'invention concerne des kits comprenant des composants destinés à mettre en oeuvre ces procédés et des instructions d'utilisation.

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