Methods of quantifying methotrexate metabolites

Details Methods of quantifying methotrexate metabolites Methods of quantifying methotrexate metabolites

C - Chemistry, Metallurgy

12

N

C12N 9/48 (2006.01) C12P 7/24 (2006.01) C12P 13/14 (2006.01) C12Q 1/26 (2006.01) C12Q 1/34 (2006.01) C12Q 1/37 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2496901

The present invention provides a method for efficiently converting methotrexate polyglutamates to methotrexate in a cellular extract by contacting the cellular extract with gamma glutamyl hydrolase under conditions suitable for efficient conversion of methotrexate polyglutamates to methotrexate.

La présente invention a trait à un procédé permettant la conversion efficace de polyglutamates de méthotrexate en méthotrexate dans un extrait cellulaire par la mise en contact de l'extrait cellulaire avec la gamma-glutamyl hydrolase dans des conditions appropriées pour une conversion efficace des polyglutamates de méthotrexate en méthotrexate.

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