Methods of refolding mammalian glycosyltransferases

Details Methods of refolding mammalian glycosyltransferases Methods of refolding mammalian glycosyltransferases

C - Chemistry, Metallurgy

12

N

C12N 9/10 (2006.01) C12P 19/00 (2006.01) C12P 21/00 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2555109

The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.

La présente invention a trait à des procédés de repliement de glycosyltransférases mammaliennes qui ont été produites dans des cellules bactériennes, et à des procédés d'utilisation desdites glycosyltransférases repliées, comprenant des mutants de glycosyltransférases qui présente une capacité améliorée de repliement. L'invention a également trait à des procédés de repliement de plusieurs glycosyltransférases dans un unique réceptacle, à des procédés d'utilisation desdites glycosyltransférases repliées, et à des mélanges réactionnels comportant les glycosyltransférases repliées.

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