Methods of screening for beta-amyloid peptide production...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/18 (2006.01) C07K 14/47 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2174632

Processing of .beta.-amyloid precursor protein (.beta.APP) is monitored by detecting the secretion of a soluble amino-terminal fragment or .beta.APP (ATF-.beta.APP) resulting from cleavage of .beta.APP at the amino-terminus of .beta.-amyloid peptide. Secretion of ATF-.beta.APP in animal models may be monitored to identify inhibitors of .beta.-amyloid production. The ATF-.beta.APP may be detected using antibodies and other specific binding substances which recognize a carboxy terminal residue on the fragment. Animals expressing the Swedish mutation of .beta.APP are described which produce abundant amounts of ATF-.beta.APP.

La maturation de la protéine précurseur de .beta.-amyloïde est surveillée par détection de la sécrétion d'un fragment amino-terminal soluble ou .beta.APP (ATF-.beta.APP) résultant de la séparation de .beta.APP au niveau de la terminaison amino du peptide .beta.-amyloïde. La sécrétion d'ATF-.beta.APP chez des modèles animaux peut être surveillée pour permettre l'identification des inhibiteurs de la production du peptide .beta.-amyloïde. L'ATF-.beta.APP peut être détectée à l'aide d'anticorps et d'autres substances de liaison spécifiques qui reconnaissent un reste terminal carboxy sur le fragment. Des animaux exprimant la mutation suédoise de .beta.APP et produisant des quantité abondantes d'ATF-.beta.APP sont également décrits.

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