Methods of screening for binding interaction using sets of...

C - Chemistry – Metallurgy – 40 – B

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C40B 30/04 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2686211

The present invention relates to methods for screening for binding interactions using multiple sets of microparticles, wherein said set has the same identifiable characteristic and wherein one of more sets comprise subsets of microparticles and said subset presents at least one unique probe that acts as a binding partner for a target molecule in a biological sample. In particular, the invention provides for methods of detecting tissue -typing antigens in donor tissue or recipient tissue using these multiple sets of microparticles.

La présente invention concerne des procédés de criblage d'interactions de liaisons utilisant de multiples ensembles de microparticules, lesdits ensembles ayant la même caractéristique identifiable. Un ou plusieurs ensembles comprennent des sous-ensembles de microparticules, chaque sous-ensemble présentant au moins une sonde unique qui agit comme un partenaire de liaison pour une molécule cible dans un échantillon biologique. En particulier, l'invention propose des procédés de détection d'antigènes de typage tissulaire dans un tissu de donneur ou un tissu de receveur qui utilisent ces multiples ensembles de microparticules.

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