Methods of screening for introduction of dna into a target cell

Details Methods of screening for introduction of dna into a target cell Methods of screening for introduction of dna into a target cell

C - Chemistry, Metallurgy

12

N

C12N 15/65 (2006.01) C12N 9/02 (2006.01) C12P 21/06 (2006.01) C12Q 1/04 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2440560

The present invention provides methods of introducing a polynucleotide into a target cell, wherein the method employs a light generating protein coding sequence acting as a reporter. An important advantage of the methods described herein is that drug resistant target cells or target cells having no useful auxotrophic markers can be effectively transformed. The present invention also includes transformed cells produced by the methods described herein. Also described are light generating protein coding sequence modifications, a variety of vectors, and methods of using the transformed cells of the present invention.

Cette invention concerne des méthodes servant à introduire un polynucléotide dans une cellule cible, et faisant appel à une séquence codante de protéine de génération de lumière agissant comme rapporteur. Un avantage important de ces méthodes se caractérise par ce que les cellules cibles résistant aux médicaments ou les cellules cibles ne comportant aucun marqueur auxotrophe utile peuvent être efficacement modifiées. Cette invention concerne également des cellules modifiées obtenues par ces méthodes, de même que des modifications de séquences codantes de protéine de génération de lumière, une variété de vecteurs ainsi que des méthodes d'utilisation des cellules modifiées de la présentes invention.

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