Methods of selecting cell clones

Details Methods of selecting cell clones Methods of selecting cell clones

G - Physics

01

N

G01N 33/569 (2006.01)

Patent

CA 2662399

The invention describes novel methods for selecting cell clones which produce high amounts of protein of interest. In one method the amount of protein is measured before the cells are passaged for the first time. In another method a high throughput automated platform is used under sterile environment conditions with class A particle load of less than 100 particles per m3.

La présente invention concerne de nouveaux procédés de sélection de clones cellulaires qui permettent la production de quantités élevées d'une protéine d'intérêt. Dans le cadre d'un procédé, la quantité de protéine est mesurée avant le premier passage des cellules. Dans le cadre d'un autre procédé, une plate-forme automatisée à rendement élevé est utilisée dans des conditions d'environnement stérile avec une charge de particules de classe A de moins de 100 particules par m3.

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