Methods of single nucleotide primer extension to detect...

Details Methods of single nucleotide primer extension to detect... Methods of single nucleotide primer extension to detect...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07K 14/47 (2006.01) C12N 15/10 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2139433

2139433 9401447 PCTABS00030 A method of determining the identity of a nucleotide base at a specific position in a nucleic acid of interest is described. The identity of the nucleotide is based on whether or not a primer is extendable in the presence of a specific substrate nucleotide. The nucleotide base to be determined lies at the 3' end immediately adjacent to the 3' end nucleotide of the primer. The primer would be extended if the substrate nucleotide is complementary to the nucleotide base in the nucleic acid of interest, and it would not if there is no said complementarity. The method used to determine a normal and mutated gene is illustrated in the figure.

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