Methods of specifying mesodermal, endodermal and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/073 (2010.01) C12N 5/0735 (2010.01) A61K 35/12 (2006.01) A61K 38/17 (2006.01)

Patent

CA 2626152

We disclose a method for producing a mesodermal or a endodermal cell from a pluripotent stem cell, the method comprising activating a Wnt signalling pathway in the pluripotent stem cell. In some embodiments, the pluripotent stem cell is in a substantially 2 dimensional configuration, such as a monolayer, for at least a portion of the time when the Wnt signalling pathway is activated.

L'invention concerne un procédé pour produire une cellule mésodermique ou endodermique à partir d'une cellule souche pluripotente, lequel procédé consiste à activer une voie de signalisation Wnt dans la cellule souche pluripotente. Selon certains modes de réalisation, cette cellule souche pluripotente se trouve dans une configuration sensiblement bidimensionnelle, telle qu'une monocouche, pour au moins une partie de la durée pendant laquelle la voie de signalisation Wnt est activée.

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