Methods of surface modification to enhance cell adhesion

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01) C12M 3/00 (2006.01) C12N 11/00 (2006.01)

Patent

CA 2537997

The current invention relates to methods of producing a surface with enhanced cell-formed surface such that at least one intermediate adhesive properties comprising treating a pre reactive group is exposed on the surface. The exposed intermediate reactive group is then reacted to create a self-assembled monolayer, comprising at least one reactive group. The self~-assembled monolayer is created non-mechanically. The reactive group is then coupled to at least one cell-adhesive molecule. The current invention also relates to cell culture devices comprising at least one oxygen-sensing compound and a cell-adhesive molecule.

La présente invention concerne des procédés de production d'une surface présentant des propriétés d'adhésion cellulaire améliorées, consistant à traiter une surface préformée de telle sorte qu'au moins un groupe réactif intermédiaire soit exposé sur la surface. Le groupe réactif intermédiaire exposé est ensuite mis à réagir afin de créer une monocouche auto-assemblée comprenant au moins un groupe réactif. La monocouche auto-assemblée est créée de manière non mécanique. Le groupe réactif est alors couplé à au moins une molécule d'adhésion cellulaire. La présente invention concerne également des dispositifs de culture cellulaire comprenant au moins un composé de détection d'oxygène et une molécule d'adhésion cellulaire.

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