Methods of terminal sterilization of biological products

A - Human Necessities – 61 – L

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Details

A61L 2/16 (2006.01) A61K 35/14 (2006.01) A61K 38/00 (2006.01) A61K 47/26 (2006.01) A61L 2/00 (2006.01) A61L 2/04 (2006.01)

Patent

CA 2254914

The invention relates to methods of sterilizing biologically active products, particularly therapeutic or prophylactic products and the compositions obtained thereby. The methods include obtaining a dried sample containing an amount of trehalose sufficient to render heat stability to the product and exposing the dried sample to heating conditions at a temperature and for a duration sufficient to substantially inactivate viruses, especially non-lipid encapsulated viruses. The drying methods include both ambient drying conditions and lyophilization. The heating conditions include any known in the art and cover a wide range of temperatures and heating times. The compositions obtained contain stable products and do not contain measurable infectious virus, particularly parvovirus.

Méthodes de stérilisation de produits biologiquement actifs en particulier de produits destinés aux traitements thérapeutiques et préventifs, et compositions ainsi obtenues. Ces méthodes consistent à obtenir un échantillon déshydraté contenant une quantité de tréhalose suffisante pour rendre au produit sa stabilité thermique, puis à exposer l'échantillon déshydraté à des conditions de chauffage comportant une température et une durée suffisantes pour inactiver dans une large mesure les virus, en particulier les virus non encapsulés par un lipide. Les méthodes de déshydratation comprennent à la fois un séchage aux conditions ambiantes et une lyophilisation. Les conditions de chauffage comprennent toutes les techniques connues et couvrent un large éventail de températures et de durées chauffage. Les compositions ainsi obtenues sont dépourvues de virus infectieux mesurables et ne contiennent en particulier pas de parvovirus.

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