Methods of testing antagonists for their abilities to affect...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2186979

Methods to test and rank substances for their abilities to affect G protein-coupled receptor activity. Specifically, these methods include testing the ability of the antagonist to increase spontaneous G protein-coupled receptor activity and to sensitize G protein-coupled receptors to agonists are described. These methods will be useful in the pharmaceutical industry for screening new drugs for their abilities to interact with G protein-coupled receptors. Reagents necessary to use this method can be supplied as part of a test kit.

Méthodes d'essai et de classement de substances en fonction de leur capacité à influer sur les activités des récepteurs couplés aux protéines G. Spécifiquement, ces méthodes comprennent la vérification de la capacité des antagonistes à accroître l'activité spontanée des récepteurs couplés aux protéines G et de sensibiliser les récepteurs couplés aux protéines G aux agonistes. Ces méthodes seront utiles dans l'industrie pharmaceutique pour cribler de nouveaux médicaments en fonction de leur capacité à interagir avec les récepteurs couplés aux protéines G. Les réactifs nécessaires pour appliquer cette méthode peuvent être offerts dans une trousse d'essai.

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