Methods of treating microbial infections in humans and animals

Details Methods of treating microbial infections in humans and animals Methods of treating microbial infections in humans and animals

A - Human Necessities

61

K

A61K 31/435 (2006.01) A61K 31/165 (2006.01) A61K 31/221 (2006.01) A61K 31/335 (2006.01) A61K 31/341 (2006.01) A61K 31/365 (2006.01) A61K 31/381 (2006.01) A61K 31/39 (2006.01) A61K 31/4409 (2006.01)

Patent

CA 2491573

A method of treating a subject with a microbially-based infection, comprising the administration of a compound to the subject. The compound is able to decrease ATP levels in the microbe by at least 10% compared to controls after 24 hours in an in vitro test, without killing mammalian cells during the same time period. The decrease in ATP levels is measured by: (1) removing the cells from the testing location and putting them on ice; (2) harvesting the cells at 4 degrees C by centrifugation and disrupting it with bead-beating in an ATP extraction buffer; (3) removing cellular debris by centrifugation at 4 degrees C, leaving an ATP-containing supernatant; (4) measuring the amount of ATP present in the supernatant by a bioluminescence assay at 4 degrees C.

L'invention concerne une méthode de traitement d'un sujet souffrant d'une infection microbienne, consistant à administrer un composé au sujet. Le composé peut réduire les niveaux d'ATP dans le microbe d'au moins 10% par rapport aux groupes témoins après 24 heures dans un test in vitro, sans tuer de cellules mammifères pendant la même période temporelle. La réduction des niveaux d'ATP est mesurée par (1) retrait des cellules de l'emplacement de test et par mise en place de celles-ci sur de la glace; (2) par culture des cellules à 4 ·C par centrifugation et interruption par battement des billes dans un tampon d'extraction d'ATP; (3) par élimination des débris cellulaires par centrifugation à 4 ·C, conservant un surnageant contenant l'ATP; (4) et par mesure de la quantité d'ATP présente dans le surnageant par un dosage bioluminescent à 4 ·C.

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