Methods of using improved polymerases

Details Methods of using improved polymerases Methods of using improved polymerases

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/04 (2006.01) C07K 5/00 (2006.01) C07K 14/00 (2006.01) C07K 14/195 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2468838

This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

L'invention concerne des procédés permettant de séquencer et d'effectuer des réactions de la polymérase au moyen d'une génération améliorée de polymérases d'acide nucléique. Les améliorations résident dans la fusion d'un domaine de liaison d'acide nucléique non spécifique de la séquence avec l'enzyme de telle sorte que la processivité de la polymérase soit augmentée.

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