Methods of using semiconductor nanocrystals in bead-based...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2403708

Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide and/or an amplification product therefrom are provided. The methods comprise contacting a sample suspected of containing the target polynucleotide with a polynucleotide that can bind specifically thereto; this polynucleotide is conjugated to a substrate, preferably an encoded bead conjugate. An amplification reaction can first be used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay for the target polynucleotide. An amplification product detection complex and method of forming the same are also provided. The methods are particularly useful in multiplex settings where a plurality of targets are present. Amplification product assay complexes and amplification product assay arrays are also provided, along with methods of forming the same. Kits comprising reagents for performing such methods are also provided.

L'invention concerne des procédés, des compositions et des articles fabriqués destinés à tester un échantillon contenant un polynucléotide cible et/ou un de ses produits d'amplification. Les procédés consistent à mettre en contact un échantillon suspecté de contenir le polynucléotide cible avec un polynucléotide qui peut s'y lier spécifiquement, ce polynucléotide étant conjugué à un substrat, de préférence un conjugué codé sur bille. On peut utiliser en premier une réaction d'amplification afin de produire le produit d'amplification à partir du polynucléotide cible afin qu'il puisse être utilisé pour tester indirectement le polynucléotide cible. L'invention concerne aussi un complexe de détection de produit d'amplification et son procédé de fabrication. Ces procédés sont particulièrement utiles dans les techniques multiplex dans lesquelles de nombreuses cibles sont présentes. L'invention concerne encore des complexes de test de produit d'amplification et des matrices de tests de produit d'amplification, ainsi que leurs procédés de fabrication. Elle concerne enfin des nécessaires comprenant des réactifs destinés à réaliser de tels procédés.

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