Methods related to modified glycans

Details Methods related to modified glycans Methods related to modified glycans

C - Chemistry, Metallurgy

07

H

C07H 1/06 (2006.01) C07H 1/00 (2006.01)

Patent

CA 2743181

Among other things, the present disclosure provides methods for enriching, identifying, and/or quantifying unusually modified glycans (e.g., phosphorylated glycans, sulfated glycans, and/or multi- acetylated glycans). In many embodiments, methods comprise providing a glycan preparation from which sialic acids have been released; subjecting the sialidase-treated glycan preparation to a separation technique that separates glycans based on charge-to-mass ratio; and quantifying the charged products using at least one quantification standard.

Entre autres choses, la présente invention porte sur des procédés pour enrichir, identifier et/ou quantifier des glycanes inhabituellement modifiés (par exemple, glycanes phosphorylés, glycanes sulfatés et/ou glycanes multi-acétylés). Dans de nombreux modes de réalisation, les procédés comprennent la fourniture d'une préparation de glycane à partir de laquelle des acides sialiques ont été libérés; le fait de soumettre la préparation de glycane traité par sialidase à une technique de séparation qui sépare les glycanes sur la base du rapport charge sur masse; et la quantification des produits chargés à l'aide d'au moins une norme de quantification.

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