Methods to activate or block the hla-e/qa-1 restricted cd8+...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 17/02 (2006.01) C12N 5/0784 (2010.01) A61K 38/08 (2006.01) A61K 39/00 (2006.01) A61K 39/385 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) A61P 37/06 (2006.01) C07K 14/74 (2006.01) C07K 16/18 (2006.01) C07K 16/28 (2006.01) C12Q 1/02 (2006.01) G01N 33/567 (2006.01) C07K 14/725 (2006.01)

Patent

CA 2678493

Methods are provided for inhibiting or enhancing down- regulation of an antigen-activated HLA-E+ T cell by an HLA-E-restricted CD8+ T cell comprising contacting the HLA-E* T cell and CD8 + T cell with an agent which inhibits or enhances, respectively, binding between (i) T cell receptor (TCR) on the surface of the CD8+ T cell and (ii) a self peptide presented by HLA-E on the surface of the HLA-E+ T cell, thereby inhibiting or enhancing, respectively, down-regulation of the antigen- activated HLA-E+ T cell. Compositions comprising agents which inhibit or enhance/activate, respectively, binding between (i) T cell receptor (TCR) on the surface of a CD8+ T cell and (ii) a self peptide presented by HLA-E on the surface of a HLA-E+ T cell, and assays for identifying such agents, are provided.

L'invention concerne des méthodes destinées à inhiber ou renforcer la régulation à la basse d'un lymphocyte T HLA-E+ activé par antigène au moyen d'un lymphocyte T CD8+ restreint par HLA-E, consistant à mettre le lymphocyte T HLA-E+ et le lymphocyte T CD8+ en contact avec un agent inhibant ou renforçant, respectivement, la liaison entre (i) le récepteur de lymphocytes T (TCR) sur la surface du lymphocyte T CD8+ et (ii) un auto-peptide présenté par HLA-E sur la surface du lymphocyte T HLA-E+, ce qui permet d'inhiber ou de renforcer, respectivement, la régulation à la baisse du lymphocyte T HLA-E+ activé par antigène. L'invention concerne également des compositions renfermant des agents qui inhibent ou renforcent/activent, respectivement, la liaison entre (i) le récepteur de lymphocytes T (TCR) sur la surface d'un lymphocyte T CD8+ et (ii) un auto-peptide présenté par HLA-E sur la surface d'un lymphocyte T HLA-E+, ainsi que des essais pour l'identification de ces agents.

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