Methods to screen herbicidal compounds utilizing air...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 15/52 (2006.01) C12Q 1/25 (2006.01)

Patent

CA 2329690

The present invention discloses methods to screen chemicals for herbicidal activity using recombinantly produced enzymes having AIR synthetase activity, and the use thereby to identify herbicidal chemicals to suppress the growth of undesired vegetation. Furthermore, the present invention provides methods for the development of herbicide tolerance in plants, plant tissues, plant seeds, and plant cells using genes encoding enzymes having AIR synthetase activity, and methods of using such transgenic plants to selectively suppress weed growth in crop fields.

L'invention concerne des procédés de criblage de composés chimiques, destinés à rechercher l'activité herbicide de ceux-ci, au moyen d'enzymes produits de manière recombinée et possédant une activité de 5'-phosphoribosyl-5-amino-imidazole synthétase (AIR synthétase). Elle concerne également l'utilisation de ces procédés pour identifier des composés chimiques herbicides destinés à supprimer la croissance de la végétation indésirable. En outre, l'invention concerne des procédés visant à rendre tolérants aux herbicides, des plantes, tissus végétaux, graines et cellules végétales, et mettant en oeuvre des gènes codant pour des enzymes possédant une activité AIR synthétase. Elle concerne enfin des procédés d'utilisation de telles plantes transgéniques, destinés à supprimer de manière sélective la croissance des mauvaises herbes dans les champs de culture.

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