C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/34 (2006.01) C12N 9/22 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2429971
Modified hairpin-forming oligonucleotide to continously assess nucleotide cleavage by enediynes and other nucleic acid cleavage agents are provided. These oligonucleotide probes, which are also referred to herein as "molecular break lights", are also useful for continuous assessment of protection of nucleotides from cleavage agents. Probes according to the present invention are useful in assays; improved assays, including multiplexed assays, utilizing such pairs of molecules or moieties; and assay kits that include such pairs. Methods of using the probes are also provided.
L'invention concerne un oligonucléotide modifié formant une tête d'épingle destiné à évaluer de façon continue le clivage nucléotidique par des enediynes ainsi que d'autres agents de clivage d'acide nucléique. Ces sondes oligonucléotidiques, lesquelles sont également appelées ici "lumières de décomposition moléculaire" sont également utiles à l'évaluation continue de la protection de nucléotides contre des agents de clivage. Les sondes selon la présente invention sont utiles dans des dosages; des dosages améliorés, notamment des dosages multiplexés, utilisant ces paires de molécules ou fractions; et des matériels de dosage contenant lesdites paires. L'invention concerne également des méthodes d'utilisation des sondes.
Prudent James
Thorson Jon S.
Fetherstonhaugh & Co.
Prudent James
Sloan-Kettering Institute For Cancer Research
Thorson Jon S.
LandOfFree
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