Methods using pores

Details Methods using pores Methods using pores

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2629009

The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

L'invention concerne un procédé d'identification d'un nucléotide individuel, comprenant (a) la mise en contact du nucléotide avec un pore de protéine transmembrane de sorte que le nucléotide interagit avec le pore et (b) la mesure du courant traversant le pore pendant l'interaction ce qui permet de déterminer l'identité du nucléotide. L'invention concerne également un procédé de séquençage de séquences d'acides nucléiques et des kits associés.

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