Methylated nucleotide regulation of upa gene expression

Details Methylated nucleotide regulation of upa gene expression Methylated nucleotide regulation of upa gene expression

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A61K 31/7115 (2006.01) A61P 35/04 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2415723

The present invention provides a method of regulating the expression of the urokinase type plasminogen activator ("uPA") gene in a mammalian cell comprising the steps of: identifying a target nucleotide sequence in a gene of interest, obtaining a methylated antisense oligonucleotide sequence complementary to the target sequence, said oligonucleotide sequence having 5 methylated cytosine in place of unmethylated cytosine in the region complementary to the CpG region in the target nucleotide sequence, and exposing the cell to copies of the oligonucleotide sequence under suitable conditions, such that the oligonucleotide sequence enters the nucleus and interacts with the target sequence to promote methylation of the target nucleotide sequence.

L'invention concerne un procédé servant à réguler l'expression du gène de l'activateur de plasminogène de type urokinase ("uPA") dans une cellule mammifère, ce qui consiste à: identifier une séquence de nucléotides cible dans un gène, obtenir une séquence d'oligonucléotides antisens méthylée complémentaire à la séquence cible, ladite séquence d'oligonucléotides possédant cytosine 5 méthylée à la place de cytosine non méthylée dans la zone complémentaire à la zone CpG de la séquence de nucléotides cible et exposer cette cellule à des copies de la séquence d'oligonucléotides dans des conditions appropriées, de sorte que cette séquence d'oligonucléotides pénètre dans le noyau et entre en interaction avec la séquence cible afin de promouvoir la méthylation de la séquence de nucléotides cible.

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