C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12N 1/20 (2006.01) C12N 15/63 (2006.01) C12N 15/74 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/00 (2006.01)
Patent
CA 2364043
The present invention relates to a method of producing a recombinant peptide, a recombinant protein, or a product from metabolic engineering using a genetically modified methylotrophic bacterium, and more particularly to Methylobacterium extorquens ATCC 55366. The method comprises introducing an expression vector into the methylotrophic bacterium, the expression vector comprising a polynucleotide sequence, encoding for a peptide or protein, or allowing for production of a product from metabolic engineering under the control of a regulated promoter. The method also comprises growing the genetically modified methylotrophic bacterium in a minimal salts medium lacking organic sugars and containing methanol. A metal ion may be used for regulating the expression of the polynucleotide sequence by the promoter.
Figueira Marianne M.
Laramee Louise
Miguez Carlos B.
Murrell J. Colin
Murrell J. Colin
National Research Council Of Canada
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
The University Of Warwick
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