Mhc-antigen complexes for detecting and purifying...

Details Mhc-antigen complexes for detecting and purifying... Mhc-antigen complexes for detecting and purifying...

C - Chemistry, Metallurgy

07

K

C07K 19/00 (2006.01) C07K 14/725 (2006.01) C07K 14/74 (2006.01) C12N 9/00 (2006.01) C12Q 1/24 (2006.01) G01N 33/532 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2212747

T cells are specifically labeled according to their antigen receptor by binding of a multimeric binding complex. The complex is prepared with major histocompatibility complex protein subunits having a homogeneous population of peptides bound in the antigen presentation site. The multimeric MHC-antigen complex forms a stable structure with T cells, thereby allowing for the labeling, identification and separation of specific T cells.

Des lymphocytes T sont marqués, de manière spécifique, selon leur récepteur d'antigène par la liaison d'un complexe de liaison multimère. Ce complexe est préparé avec des sous-unités de protéines du complexe majeur d'histocompatibilité (MHC), comportant une population homogène de peptides liés dans le site de présentation à l'antigène. Le complexe antigènes/MHC multimère forme une structure stable avec les lymphocytes T, ce qui permet le marquage, l'identification et la séparation de lymphocytes T spécifiques.

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