Micelle protection assay

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2223047

Methods and compositions for selectively detecting analytes in a homogeneous assay, a heterogeneous assay, or a mixture of the two by contacting a labeled probe:analyte complex with one or more amphiphiles. The invention is also useful for increasing the signal to noise ratio when used in conjunction with other assay systems. In preferred embodiments, the analyte and probe are nucleic acids or proteins.

L'invention porte sur des procédés et des compositions servant à la détection sélective d'analytes dans des dosages homogènes, des dosages hétérogènes ou dans un mélange des deux. Le procédé consiste à mettre en contact un complexe sonde marquée/analyte avec un ou plusieurs amphiphiles. L'invention sert également à amplifier le rapport signal/bruit lorsqu'elle est utilisée conjointement avec d'autres systèmes de dosage. Dans des modes de réalisations préférées, les analytes et sondes sont des acides nucléiques ou des protéines.

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