Micro-organism culture method

Details Micro-organism culture method Micro-organism culture method

C - Chemistry, Metallurgy

12

N

C12N 1/36 (2006.01) C02F 3/34 (2006.01) C12N 1/26 (2006.01)

Patent

CA 2184610

The invention concerns an accelerated microorganism, culture method for decomposing harmful substances. For this purpose, according to the invention the cultivation, mutagenic treatment and stabilisation of the microorganisms are carried out simultaneously in a mutation fermenter containing a nutrient-rich basic medium (basic medium 1); selection is carried out in a selection fermenter containing a basic medium which is low in nutrients and is enriched with the harmful substance (basic medium 2); small amounts of biomass released from the nutrient-rich basic medium (basic medium 1) of the mutation fermenter are continuously removed from the mutation fermenter and transferred to the selection fermenter: small amounts of biomass released from the basic medium which is low in nutrients (basic medium 2) of the selection fermenter are removed from the selection fermenter and transferred to the mutation fermenter: the entire process is carried out continuously in alternating selection and mutation phases, in the manner of a repeated cycle, and the mutation fermenter and the selection fermenter are associated with computerized control circuits by means of which the fermentation parameters can be adjusted independently of one another in both fermenters.

L'invention concerne un procédé de culture accélérée de micro-organismes utile pour décomposer des polluants. A cet effet, la culture, le traitement mutagène et la stabilisation des micro-organismes sont effectués simultanément dans un fermentateur de mutation qui contient un milieu de base (milieu de base 1) riche en substances nutritives. La sélection est effectuée dans un fermentateur de sélection qui contient un milieu de base (milieu de base 2) pauvre en substances nutritives et enrichi en polluant. On prélève en continu de petites quantités de biomasse dans le fermentateur de mutation, on en élimine le milieu de base (milieu de base 1) riche en substances nutritives que contient le fermentateur de mutation et on les transfère au fermentateur de sélection. On prélève en continu de petites quantités de biomasse dans le fermentateur de sélection, on en élimine le milieu de base (milieu de base 2) pauvre en substances nutritives que contient le fermentateur de sélection et on les transfère au fermentateur de mutation. Tout le procédé se déroule ainsi en continu en phases alternantes de sélection et de mutation d'un cycle répétitif. Des circuits informatisés de régulation associés d'une part au fermentateur de mutation et d'autre part au fermentateur de sélection règlent les paramètres de fermentation dans les deux fermentateurs indépendamment l'un de l'autre.

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