Micro-organisms with glycosylation modulating action of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01)

Patent

CA 2414841

The invention concerns micro-organism strains, in particular of lactic acid bacteria, having a glycosylation modulating effect of intestinal cell surface. The invention also concerns a method for selecting micro-organism strains, in particular of lactic acid bacteria, which consists in measuring the average fluorescence intensity variation of HT29-MTX cells incubated in the presence of a lectin coupled with a fluorochrome after being in contact with the supernatant of the strain concerned. Said lactic acid bacteria strains can be used, optionally in the form of their active fraction, for preparing food compositions or medicines or food supplements, modulating glycosylation of glycoproteins of intestinal epithelial cells.

L'invention a pour objet les souches de microorganismes, en particulier de bactéries lactiques, ayant un effet modulateur de bactéries lactiques, ayant un effet modulateur de la glycosylation de surface des cellules intestinales. L'invention a également pour objet une méthode de sélection des souches de microorganismes, en particulier de bactéries lactiques, dans laquelle est mesurée la variation de l'intensité de fuorescence moyenne de cellules HT29- MTX incubées en présence d'une lectine couplée à un fluorochrome après avoir été en contact avec le surnageant de chaque souche étudiée. Ces souches de bactéries lactiques peuvent être utilisées, éventuellement sous forme de leur fraction active, pour la préparation de compositions alimentaires ou médicaments ou compléments alimentaires, modulant la glycosylation des glycoprotéines des cellules epithéliales intestinales.

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