Micro well array and method of sealing liquid using the...

G - Physics – 01 – N

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G01N 35/00 (2006.01) B01L 3/00 (2006.01) B23K 20/10 (2006.01) B29C 65/00 (2006.01) B29C 65/08 (2006.01) B29C 65/58 (2006.01) B29C 65/78 (2006.01) C12M 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01) G01N 33/48 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2422569

A micro well array and a method of sealing liquid by using the micro well array; the micro well array, comprising a container having a plurality of independent wells disposed, therein, in array shape and a cover capable of covering the container, characterized in that the area of each well near the edge part thereof forms a projected part higher than the peripheral area thereof; the method, comprising the steps of injecting the liquid into the wells by an amount exceeding the volume of the wells after depositing to extrude the excess liquid from the wells so that air does not almost remain in the wells, whereby minute amount of specimen is allowed to react with reagent in a very small space and a fluorescence forming signals can be taken out efficiently.

Cette invention a trait à un ensemble de micro-coupelles, et au procédé correspondant, permettant d'enfermer hermétiquement des liquides. Cet ensemble est constitué d'un contenant dans lequel sont disposées, en réseau, plusieurs coupelles indépendantes, et d'un couvercle recouvrant le contenant. Cet ensemble se caractérise par le fait que la partie proche du rebord de chaque coupelle se trouve en saillie par rapport au pourtour de celle-ci. Le procédé selon l'invention consiste à injecter dans les coupelles davantage de liquide que celles-ci ne peuvent en contenir, puis, une fois celles-ci remplies, à en expulser le liquide en excès, de sorte qu'il n'y reste pas d'air. De faibles quantités d'échantillon peuvent, de la sorte, réagir avec un réactif dans un espace très réduit et il est possible de déceler, de manière satisfaisante, les signaux de fluorescence produits.

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