Microarray methods utilizing semiconductor nanocrystals

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2400379

The present invention provides a number of different methods for conducting assays with different types of addressable arrays utilizing semiconductor nanocrystals to enhance detection. The invention includes methods utilizing semiconductor nanocrystals with nucleic acid, protein and tissue arrays, for example. By utilizing various useful aspects of semiconductor nanocrystals, the invention also provides a variety of different options for conducting multiplexed assays. Additionally, detection methods involving counting of individual complexes that include semiconductor nanocrystals are provided which can be utilized to expand the dynamic range of detection.

La présente invention concerne plusieurs techniques différentes permettant de réaliser des analyses avec différents types de jeux d'échantillons adressables utilisant des nanocristaux semi-conducteurs afin d'améliorer la détection. L'invention concerne également des techniques utilisant des nanocristaux semi-conducteurs avec, être autres, des jeux d'échantillons d'acides nucléiques, de protéines et de tissus. En utilisant plusieurs aspects utiles des nanocristaux semi-conducteurs, l'invention présente en outre plusieurs choix pour réaliser des analyses multiplexées. L'invention concerne aussi des techniques de détection consistant à compter individuellement les complexes comprenant des nanocristaux semi-conducteurs, pouvant s'utiliser afin d'étendre la gamme dynamique de détection.

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