Microassay on a card

Details Microassay on a card Microassay on a card

G - Physics

01

N

G01N 33/94 (2006.01) B01L 3/00 (2006.01) G01N 21/78 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01) G01N 33/558 (2006.01) G01N 33/577 (2006.01) G01N 21/29 (2006.01) G01N 21/76 (2006.01)

Patent

CA 2091255

A microassay card (10) includes an upper layer (12) containing wells (14) for receiving a liquid sample (16). A second layer (20) of the card (10), beneath the first layer (12), includes a supporting surface (223) bound to a reactive spe- cies (114). A third layer (26) includes a superabsorbent sup- port (26) impregnated with an indicator (228). Typically, the indicator (228) is a substrate (228) for an enzyme (16), such as a reduced dye precursor and a source of hydrogen perox- ide necessary for the action of the enzyme (116), upon the substrate to cause a spectral change in the absorbent layer (26). By selecting the structure of the first (12) and second (20) layers, the card (10) can be formatted for a displacement assay or a competitive assay. The microassay card (10) of the present invention is particularly useful for drug testing.

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