G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/53 (2006.01) G01N 33/50 (2006.01) G01N 33/541 (2006.01) G01N 33/569 (2006.01) G01N 33/571 (2006.01)
Patent
CA 1083956
Abstract Immunoassay for determining the presence of microbial antibodies in mammalian serum which comprises the steps of: A. Providing a reaction mixture comprising a buffered aqueous medium; serum to be sampled; antigen derived from said microbes and anti- antibody to the serum to be tested, said antigen or anti-antibody bearing a detectable label; B. Incubating said reaction mixture at from about 4 to 45°C. for from about 24 to 2 hours at a pH of from about 6.5 to 8.5 to form an antigen- antibody conjugate when said microbial antibody is present in said serum sample; and C. Determining the level of said label as a measure of the presence of said antigen-antibody conjugate; characterized in that the volume ratio of anti-antibody to serum sample is adjusted to a value sufficient to form an agglutinate but less than required to effectuate precipitation of the antigen-anti- body conjugate.
287947
Gomez Magdalena U.
Mondabaugh Susan M.
Gowling Lafleur Henderson Llp
Hoffmann-La Roche Limited
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