C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) C12Q 1/66 (2006.01) G01N 33/52 (2006.01) G01N 33/84 (2006.01)
Patent
CA 2572608
The present invention is directed to compositions and methods for single step extraction and detection of ATP levels from microbial cells. The disclosed compositions are formulated to efficiently elicit bioluminescent detection of ATP among a broad variety of different microorganisms using a common single- step reagent composition. Additional luminescence-based methods are provided for identifying other useful extracting agents or for screening compounds for their pharmaceutical or biological effects on microbial cells.
La présente invention concerne des compositions et des méthodes de détection et d'extraction en une seule étape de niveaux d'ATP à partir de cellules microbiennes. Les compositions de cette invention sont préparées de manière à éliciter efficacement la détection bioluminescente d'ATP parmi une large variété de micro-organismes différents, à l'aide d'une composition de réactifs à étape unique commune. D'autres méthodes reposant sur la luminescence permettent d'identifier d'autres agents d'extraction utiles ou de cribler des composés à la recherche de leurs effets pharmaceutiques ou biologiques sur des cellules microbiennes.
Butler Braeden
Fan Frank
Wood Keith V.
Cassan Maclean
Promega Corporation
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