Microbial degradation of trichloroethylene

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C02F 3/34 (2006.01) C12N 1/20 (2006.01) C12N 9/02 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12N 15/78 (2006.01)

Patent

CA 1316860

ABSTRACT This invention relates to a method for the microbial degradation of trichlorethylene by treating trichloroethylene with Pseudomonas mendocina KR-1 or Pseudomonas putida Y2101 or a microorganism host cell that contains a recombinant plasmid. The recombinant plasmid contains toluene monooxygenase genes from Pseudomonas mendocina KR-1. The microorganism host cell containing the recombinant plasmid must have been treated with an inducer of the toluene monooxygenase genes. The method may be applied to the treatment of loci of trichloroethylene chemical waste in water or soil. More particularly, the method may be applied to degrade trichloroethylene as it may be present as a pollutant or contaminant in water, in industrial effluents, in various land areas such as industrial sites, or in various laboratory or commercial installations.

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