Microbial expression of quasi-synthetic genes

Details Microbial expression of quasi-synthetic genes Microbial expression of quasi-synthetic genes

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.3

C12N 15/00 (2006.01) C07K 14/61 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1164375

ABSTRACT Described are methods and means for the construc- tion and microbial expression of quasi synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illus- trative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.

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